Phagolysosome formation in normal and colchicine-treated macrophages.
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چکیده
منابع مشابه
Phagolysosome formation in normal and colchicine-treated macrophages
Intracellular lysosomal fusion has been evaluated in cultivated mouse peritoneal macrophages by measurement of transfer of acid phosphatase to polyvinyltoluene (PVT)-containing phagolysosomes. Enzyme transfer was found to be directly and significantly related to the uptake of PVT and to be independent of time allowed for phagolysosome formation over time periods of 15 min to 18 h. In addition, ...
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Intravascular pH was measured within the lysosomes and newly formed phagosomes in cultured mouse peritoneal macrophages. The kinetics of pH change in both vacuolar systems was quantitatively determined within a large cell population by fluorescence spectroscopy. Additionally, pH changes within individual phagosomes were followed semiquantitatively using indicator dyes. Two novel findings were m...
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Purpose The eye is an immune-privileged microenvironment that has adapted several mechanisms of immune regulation to prevent inflammation. One of these potential mechanisms is retinal pigment epithelial cells (RPE) altering phagocytosis in macrophages. Methods The conditioned media of RPE eyecups from eyes of healthy mice and mice with experimental autoimmune uveitis (EAU) were used to treat ...
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Cryptococcus neoformans resides in an acidic phagolysosome of human macrophages.
Recently, we demonstrated that human monocyte-derived macrophages (MDM) treated with chloroquine or ammonium chloride had markedly increased antifungal activity against the AIDS-related pathogen Cryptococcus neoformans. Both of these agents raise the lysosomal pH, which suggested that the increased antifungal activity was a function of alkalinizing the phagolysosome. Moreover, there was an inve...
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ژورنال
عنوان ژورنال: Journal of Experimental Medicine
سال: 1975
ISSN: 0022-1007,1540-9538
DOI: 10.1084/jem.142.4.903